Coding

Part:BBa_K160000:Design

Designed by: RAUL CUERO   Group: iGEM08_Prairie_View   (2008-10-25)


Cl Channel


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 208
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 208
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 208
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 208
    Illegal PstI site found at 220
    Illegal AgeI site found at 1192
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 364
    Illegal SapI.rc site found at 412


Design Notes

The gene orginates from Eukaryotic cells, so cDNA was codon optimized for expression in E.coli


Source

Gene was isolated from kidney microsomes of Bos taurus (cattle).

References